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Testosterone is produced by the testes from the onset of puberty throughout adulthood under the influence of Adrenocorticotropic Hormone (ACTH). Sex hormones are manufactured from cholesterol which is converted to Pregnenolone by the ACTH. High levels of ACTH therefore stimulate greater manufacture of testosterone from cholesterol.

Testosterone is indeed produced in large quantities in the testes, and androgens (the parent hormones that give rise to testosterone) are actually produced by two small glands next to the kidneys, the adrenals, in both men and women.

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