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Laser Acupuncture Pen Formation of a connective tissue “whorl” with needle rotation. Rat subcutaneous connective tissue became dissected and placed in physiological buffer below a dissecting microscope. An acupuncture needle changed into inserted via the tissue and steadily circled. Numbers zero through 7 imply numbers of needle revolutions. A seen whorl of connective tissue may be visible with as low as one revolution of the needle. B: Scanning electron microscopy imaging of reusable gold (left) and disposable chrome steel (proper) acupuncture needles. unique magnification, 350×. C,D: Scanning electron microscopy of gold (C) and stainless-steel (D) needles. original magnification, 3,500×. The floor of the gold needle is visibly rougher than that tailor-made stainless steel. Scale bars = 2.5 mm in A, 100 µm in B, 10 µm in C,D.